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Alanine aminotransferase (ALT)
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Alanine aminotransferase (ALT)

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Alanine aminotransferase (may be referred to as AlАt, ALT, GPT) is an enzyme belonging to the group of transaminases or transferases. It is a catalyst involved in nitrogen exchange reactions. Thanks to him, the transport of amino groups for the production of amino acid reserves is carried out.

Most of these biochemical processes are carried out in the liver cells. However, this enzyme is also present in the pancreas, myocardium, skeletal muscles, and kidneys, albeit in much smaller quantities. The entry of ALT into the blood occurs when the cells of these organs are damaged.

Referral for ALT analysis can be given by one of the following specialists:
  • gastroenterologist;
  • therapist;
  • surgeon;
  • endocrinologist;
  • infectious disease specialist;
  • general doctor;
  • hematologist.

Potential blood donors must take an ALT test.

In the clinic MC "Dobrobut", located in Kyiv, you can go through the procedure for taking the material and quickly get your hands on the results. If necessary, you will receive detailed advice.

Service Description

The cost of the ALT analysis procedure includes:

  • a sampling of biomaterial (mainly venous blood, capillary blood can be used);
  • vacutainer tubes (vacuum systems for blood sampling) with a gel phase as a preservative without anticoagulant;
  • performing a UV kinetic test;
  • registration of the results of indicators in units of measurement "U/l".

At the Dobrobut MC clinic, biomaterial sampling is carried out in the comfortable conditions of a modern laboratory. The experienced staff of one of the best clinics in Ukraine performs all manipulations quickly and painlessly.

We will take care of your well-being, even if you had a negative experience of similar procedures before. We work tactfully with patients of all ages.

How is the study going

The patient needs to understand that many factors can affect the reliability of the results. Excessive physical activity is one of them, so the day before you should refuse to visit the gym or do hard physical work. Also, the level of indicators is affected by the intake of drugs:

  • oral contraceptives;
  • steroids;
  • choleretics;
  • antitumor agents;
  • immunosuppressants;
  • psychotropic drugs.

If you are taking any of these medicines, tell your doctor. There are also dietary supplements, which can also give an increased rate of alanine aminotransferase, injections and an abundance of fatty foods in the daily diet can affect.


Note:

  • It is necessary to exclude alcohol from the diet 5 days before donating blood.
  • Do not smoke 30 minutes before blood sampling.
  • You can drink water.

The material is taken on an empty stomach in the morning. At least 8 hours must have elapsed since the last meal. Blood is taken from a vein.

In the laboratory in Kyiv, the activity of alanine aminotransferase (ALT) in serum is determined using a UV kinetic test. This test is especially relevant for liver pathologies. The analysis is based on 2 stages of work with the analyte: the enzymatic transformation of the corresponding analyte, and then the oxidation of the resulting products with reagents. The rate of oxidation is proportional to the concentration of the analyte. The concentration in the UV method is determined photometrically at a wavelength of 340 nm. During incubation, the kinetic curves should reach a plateau phase.

What do you get from an alanine aminotransferase test?

For adults (over 18 years old), the level of AsAt / ALT is, respectively:

  • women - up to 34 U / l;
  • men - up to 45 U / l.

Often the level of ALT in the blood test is compared with the indicators of AST (AsAt), the so-called de Ritis coefficient. Normally, the ratio should be 0.91–1.752. However, it makes sense to calculate the coefficient only if one of the indicators has gone beyond the reference values.

If the coefficient is below 1, this may be a signal of damage to hepatocytes (liver cells). With indicators above 2 - suspicion of cardiovascular pathology.

It should be remembered that the interpretation of the test results is carried out in conjunction with the indicators of other tests and diagnostic studies. It is wrong to conclude that ALT shows only based on a single result. There are states when an increase or decrease in values ​​is the norm. For example, in the first trimester of pregnancy, a slight increase in indicators is the norm. Also, the level of ALT significantly depends on age - the younger the child, the higher it is. This is due to the peculiarities of the flow of biochemical processes in the body.

How to make an appointment for an ALT blood test, the price of the service

You just need to call in advance to find out the working hours of the laboratory in Ukraine. You can also find out information and the cost of analysis on our website or leave contacts for a call back from the administrator.

In the Dobrobut MC clinic, the price for ALT analysis is comparable to other similar offers in the city. We guarantee results within 1 business day. Just sign up for a consultation with a specialist!

You need to bring a referral from your doctor with you and follow the recommendations by refraining from eating, smoking, strong emotional and physical exertion.

Registration for service

Bibliography

  1. Lala V, Goyal A, Bansal P, et al. Liver Function Tests. [Updated 2021 Aug 20]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2021 Jan.
  2. Bekkelund SI. Serum alanine aminotransferase activity and risk factors for cardiovascular disease in a Caucasian population: the Tromsø study. BMC Cardiovasc Disord. 2021;21(1):29. Published 2021 Jan 13.
  3. Jia J, Yang Y, Liu F, et al. The association between serum alanine aminotransferase and hypertension: A national based cross-sectional analysis among over 21 million Chinese adults. BMC Cardiovasc Disord. 2021;21(1):145. Published 2021 Mar 19.
  4. Ahmed Z, Ahmed U, Walayat S, Ren J, Martin DK, Moole H, Koppe S, Yong S, Dhillon S. Liver function tests in identifying patients with liver disease. Clin Exp Gastroenterol. 2018 Aug 23;11:301-307.

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